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Difco nutrient broth agar plates
Nutrient Broth Agar Plates, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nutrient+broth+agar+plates/agar+broth+nutrient+plates/us12419978-138-11-10
Average 86 stars, based on 1 article reviews
nutrient broth agar plates - by Bioz Stars, 2026-09
86/100 stars

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Sterility:

Article Title: Experimental studies addressing the longevity of Bacillus subtilis spores – The first data from a 500-year experiment
Article Snippet: .. Spore survival was determined by standard colony formation assays; resuspended spores and spores stored in NaCl solutions were serially diluted in sterile distilled water, aliquots applied to nutrient broth agar plates (Difco, Detroit, MI) and colonies counted after incubation overnight at 37°C, all as described previously [ , , ]. ..

Article Title: No Effect of Microgravity and Simulated Mars Gravity on Final Bacterial Cell Concentrations on the International Space Station: Applications to Space Bioproduction
Article Snippet: .. Total cell count and spore counts (after heating for 15 min at 80°C; 50 μL sample volume per test in triplicates) were determined from appropriate dilutions in sterile buffered phosphate saline (0.7% w/v Na 2 HPO 4 × 2H 2 O, 0.3% w/v KH 2 PO 4 , 0.4% w/v NaCl pH 7.5) as colony-forming ability after incubation overnight at 37°C on nutrient broth agar plates (Difco, Detroit, MI, United States). ..

Incubation:

Article Title: Experimental studies addressing the longevity of Bacillus subtilis spores – The first data from a 500-year experiment
Article Snippet: .. Spore survival was determined by standard colony formation assays; resuspended spores and spores stored in NaCl solutions were serially diluted in sterile distilled water, aliquots applied to nutrient broth agar plates (Difco, Detroit, MI) and colonies counted after incubation overnight at 37°C, all as described previously [ , , ]. ..

Article Title: Diatom assemblages promote ice formation in large lakes
Article Snippet: Critical point drying was replaced by treatment with a solution of hexamethyl disilazane for 5 min and then air dried onto cover slips and coated with a 10-nm Au–Pd layer using a sputter coater (Hummer VI-A) before viewing with a Hitachi S2700 SEM (Hitachi High Technologies America, Inc., Dallas, TX, USA). .. Isolation and characterization of heterotrophic bacteria Plankton material collected by vertical net tows was streaked on Nutrient Broth agar plates (0.8% w/v nutrient broth (Difco, Becton Dickinson and Company, Franklin Lakes, NJ, USA), 1.2% w/v Bacto Agar (Difco)) and incubated at 4 °C in the dark for 72 h. Colonies from the plate were selected based on differences in colony morphology, and repeatedly subcultured at 4 °C so as to ensure purity of the final isolates. ..

Article Title: No Effect of Microgravity and Simulated Mars Gravity on Final Bacterial Cell Concentrations on the International Space Station: Applications to Space Bioproduction
Article Snippet: .. Total cell count and spore counts (after heating for 15 min at 80°C; 50 μL sample volume per test in triplicates) were determined from appropriate dilutions in sterile buffered phosphate saline (0.7% w/v Na 2 HPO 4 × 2H 2 O, 0.3% w/v KH 2 PO 4 , 0.4% w/v NaCl pH 7.5) as colony-forming ability after incubation overnight at 37°C on nutrient broth agar plates (Difco, Detroit, MI, United States). ..

Article Title: A combined low‐pressure hydrogen peroxide evaporation plus hydrogen plasma treatment method for sterilization − Part 2: An intercomparison study of different biological systems
Article Snippet: Funding information DFG package PlasmaDecon PAK 728, Grant numbers: AW 7/3-1, MO 2023/2-1, BA 4193/3-1; German Aerospace Center, Grant numbers: DLR-FuE-Projekt ISS LIFE, Programm RF-FuW, Teilprogramm 475; Ruhr University Bochum Low-pressure plasmas are a promising alternative tomodern sterilization processes.. As plasma is a surface process, multilayered stacks of spores are a crucial challenge to overcome.. Here, a combined process of condensed hydrogen peroxide and hydrogen plasma is analyzed for its efficacy against various spore concentrations showing a clear increase in efficacy using a combined process compared to the two steps used separately.

Isolation:

Article Title: Diatom assemblages promote ice formation in large lakes
Article Snippet: Critical point drying was replaced by treatment with a solution of hexamethyl disilazane for 5 min and then air dried onto cover slips and coated with a 10-nm Au–Pd layer using a sputter coater (Hummer VI-A) before viewing with a Hitachi S2700 SEM (Hitachi High Technologies America, Inc., Dallas, TX, USA). .. Isolation and characterization of heterotrophic bacteria Plankton material collected by vertical net tows was streaked on Nutrient Broth agar plates (0.8% w/v nutrient broth (Difco, Becton Dickinson and Company, Franklin Lakes, NJ, USA), 1.2% w/v Bacto Agar (Difco)) and incubated at 4 °C in the dark for 72 h. Colonies from the plate were selected based on differences in colony morphology, and repeatedly subcultured at 4 °C so as to ensure purity of the final isolates. ..

Bacteria:

Article Title: Diatom assemblages promote ice formation in large lakes
Article Snippet: Critical point drying was replaced by treatment with a solution of hexamethyl disilazane for 5 min and then air dried onto cover slips and coated with a 10-nm Au–Pd layer using a sputter coater (Hummer VI-A) before viewing with a Hitachi S2700 SEM (Hitachi High Technologies America, Inc., Dallas, TX, USA). .. Isolation and characterization of heterotrophic bacteria Plankton material collected by vertical net tows was streaked on Nutrient Broth agar plates (0.8% w/v nutrient broth (Difco, Becton Dickinson and Company, Franklin Lakes, NJ, USA), 1.2% w/v Bacto Agar (Difco)) and incubated at 4 °C in the dark for 72 h. Colonies from the plate were selected based on differences in colony morphology, and repeatedly subcultured at 4 °C so as to ensure purity of the final isolates. ..

Cell Characterization:

Article Title: No Effect of Microgravity and Simulated Mars Gravity on Final Bacterial Cell Concentrations on the International Space Station: Applications to Space Bioproduction
Article Snippet: .. Total cell count and spore counts (after heating for 15 min at 80°C; 50 μL sample volume per test in triplicates) were determined from appropriate dilutions in sterile buffered phosphate saline (0.7% w/v Na 2 HPO 4 × 2H 2 O, 0.3% w/v KH 2 PO 4 , 0.4% w/v NaCl pH 7.5) as colony-forming ability after incubation overnight at 37°C on nutrient broth agar plates (Difco, Detroit, MI, United States). ..

Saline:

Article Title: No Effect of Microgravity and Simulated Mars Gravity on Final Bacterial Cell Concentrations on the International Space Station: Applications to Space Bioproduction
Article Snippet: .. Total cell count and spore counts (after heating for 15 min at 80°C; 50 μL sample volume per test in triplicates) were determined from appropriate dilutions in sterile buffered phosphate saline (0.7% w/v Na 2 HPO 4 × 2H 2 O, 0.3% w/v KH 2 PO 4 , 0.4% w/v NaCl pH 7.5) as colony-forming ability after incubation overnight at 37°C on nutrient broth agar plates (Difco, Detroit, MI, United States). ..

Cell Culture:

Article Title: Antimicrobial composition for inhibiting microbial organisms in allograft and the method thereof
Article Snippet: Preparation of Staphylococcus aureus Staphylococcus aureus (Staph-A) strain NCTC 8532 [IAM 12544, R. Hugh 2605] was purchased from ATCC as a frozen stock. .. An aliquot of the frozen stock was initially cultured in Difco Nutrient Broth agar plates overnight at 37° C. Colonies of Staph-A from the plates were used to inoculate 3 mL cultures of Difco Nutrient Broth that were grown overnight at 37° C. with shaking. .. The overnight cultures were diluted to an optical density (OD600) value of 0.1 (equal to 107 CFU/mL) into fresh Difco Nutrient Broth in preparation for treating the bone allograft.

Article Title: A Mitogen-Activated Protein Kinase Pathway Is Required for Bacillus amyloliquefaciens PMB05 to Enhance Disease Resistance to Bacterial Soft Rot in Arabidopsis thaliana
Article Snippet: .. Bacillus amyloliquefaciens PMB05 and the bacterial soft rot pathogen, Pectobacterium carotovorum subsp. carotovorum Ecc17, were cultured on nutrient broth agar plates (NA, BD DifcoTM, Franklin Lakes, NJ, USA) at 28 °C for 48 h. Bacterial suspensions were then prepared and adjusted to an OD 620 at 0.3 (around 3.0 × 10 8 CFU/mL) with a sterilized phosphate buffer prior to conducting further experiments. ..

other:

Article Title: Resistance of Bacillus subtilis Spore DNA to Lethal Ionizing Radiation Damage Relies Primarily on Spore Core Components and DNA Repair, with Minor Effects of Oxygen Radical Detoxification
Article Snippet: Spore survival was determined from appropriate dilutions in distilled water as colony-forming ability after incubation overnight at 37°C on nutrient broth (NB) agar plates (Difco, Detroit, MI, USA) ( 33 , 34 , 36 ).



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